Resistance of Test Strains

A main task of hygienically working packaging machines and especially of packaging machines for low acid products (aseptical) is the partial decontamination or the sterilization of the packaging material just before its filling. In case of low acid products (aseptical) mostly hydrogen peroxide as decontaminating agent is used.For quantifying the efficiency of a device concerning the decontamination (packaging material and the device itself) challenge tests using Bacillus Subtilis–spores are carried out. The test microorganisms act as a sensor in form of a bio-indicator. The resistance of the bio-indicator plays an decisive role.An acknowledged accessible method for testing the resistance does not exist. Furthermore there is no supplier of germs, with a tested, defined resistance (in dry state) to hydrogen peroxide.Goal of the project is to develop a reliable method for measuring the resistance of germs in dry state to hydrogen peroxide.